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Trouble with genomic PCR

Trouble with genomic PCR - PCR - Polymerase Chain Reaction Forum

Trouble with genomic PCR - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 10-07-2007, 02:54 AM
Pipette Filler
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Unhappy Trouble with genomic PCR



Hello there,

We recently bought some mice strains that need genotyping. We are currently using the DNA extraction method from Promega (lysis buffer with EDTA, overnight incubation with proteinase K, isopropanol, etc, etc). It works well sometimes, but lately we are not getting any PCR products. The last time we got a very sharp standard ladder and positive controls, but my samples are are smeared. I can not even get GAPDH. My positive control ( from the company) works well, but my samples at the same concentration do not.
I used this kit before, and had no problem, but lately it has been very frustating. Any ideas? Any new genomic DNA extraction that works better?
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Old 10-07-2007, 02:25 PM
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Default Re: Trouble with genomic PCR

Hello Cintiad

I Am Using High Pure Of Roche For Extraccion Of Adn
And Work Very Well. Is Kit To Have For 100 Samles.
Need Centrifuge For Apendorf And Hot Bath.

I Hope Is Good For You

Bye For Now

Margarita
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