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#1
09-25-2007, 12:22 PM
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 Non-specific binding Hi, I have a question hope someone out there able to enlighten me. I ran a PCR using a primer pair to amplify a particular fragment of ~700bp. I also ran a negative control and a band appeared. I used a 1kb DNA ladder so that small band was between 750 and 1000bp. The amplified product is also between these 2 bands, it is what I wanted. Will this affect my product and what causes it? degenerated primers?  |
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| binding , nonspecific |
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