|Register||Search||Today's Posts||Mark Forums Read|
|PCR - Polymerase Chain Reaction Forum PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.|
| ||LinkBack||Thread Tools||Display Modes|
Hi, I have a question hope someone out there able to enlighten me.
I ran a PCR using a primer pair to amplify a particular fragment of ~700bp. I also ran a negative control and a band appeared. I used a 1kb DNA ladder so that small band was between 750 and 1000bp. The amplified product is also between these 2 bands, it is what I wanted. Will this affect my product and what causes it? degenerated primers?
|binding , nonspecific|
|Thread||Thread Starter||Forum||Replies||Last Post|
|non specific binding of FITC to erythrocytes||Elena Ronander||Immunohistochemistry Forum||1||06-15-2009 02:00 AM|
|how to reduce IgG non specific binding?||dizue||Antibody Forum||2||12-02-2008 08:42 AM|
|Calculations for specific activity||Morales, Victor||Protein Forum||0||10-02-2008 01:17 PM|
|ELISA for protein-protein binding assay||takayama||Protein Forum||1||06-27-2006 12:08 PM|
|Sequence non-specific DNA binding||EK||Protocols and Methods Forum||5||08-28-2003 02:41 AM|