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Non-specific binding

Non-specific binding - PCR - Polymerase Chain Reaction Forum

Non-specific binding - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 09-25-2007, 12:22 PM
Pipette Filler
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Default Non-specific binding



Hi, I have a question hope someone out there able to enlighten me.

I ran a PCR using a primer pair to amplify a particular fragment of ~700bp. I also ran a negative control and a band appeared. I used a 1kb DNA ladder so that small band was between 750 and 1000bp. The amplified product is also between these 2 bands, it is what I wanted. Will this affect my product and what causes it? degenerated primers?
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