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| Hallo! I was designing my primers for real-time PCR using Primer3 program where it gives out also melting temperatures. Now, I've got my primers and the comapny saent also melting temperatures and for most of them these temperatures are different. So what is the max difference in melting T allowed for primers and which t i have to use for my PCR. Highest or the average between two? Thank you already. |
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