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-   -   a problem about dna marker linked to a dominant verticillium wilt resistance in tomat (http://www.molecularstation.com/forum/pcr-polymerase-chain-reaction-forum/1809-problem-about-dna-marker-linked-dominant-verticillium-wilt-resistance-tomat.html)

turkish_scientist_f 07-23-2007 10:07 AM

a problem about dna marker linked to a dominant verticillium wilt resistance in tomat
 
Hi everybody,i am a biochemistry who works in a biotechnology labratoary on breeding plants.i am new here!

i ve been tring 2 optimize a dna marker 2 link verticillium..its a codominant marker and i need 2 detect an amplified product of approximately 1050 bp for resistant plants and 1000bp only in heterozygous and susceptible plants.How ever , although pcr works i just detect an amplified product of 1000bp even i am sure that my plants re heterozious!:wacko: i wonder that if anyone of u has worked on verticillium, and what results u got? thanks :happy:

kmunson779 07-23-2007 02:54 PM

Re: a problem about dna marker linked to a dominant verticillium wilt resistance in t
 
1000 and 1050 bp are not very different sizes... are you certain you can distinguish between these two bands on your gel?

turkish_scientist_f 07-24-2007 05:22 AM

Re: a problem about dna marker linked to a dominant verticillium wilt resistance in t
 
Of course,we can distinguish even 15bp distance in another tests..and also the people who developed that marker could get that double bands, i dont know whats wrong with mine,i need an alternative primer to test verticillium wilt resistance :(


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