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| when u have lyophilized primer (dried form), add TE buffer (pH 7) to make concentration 100 µM. Then dilute it to make 10µM working solution. This stock can directly be used for PCR. If u use 1µl of this stock in PCR mix (25µl) u are using 0.4 µM of primer in final mix. Hope i have solved ur problem. |
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| Concentration dilution series | dolphin | Mass Spectrometry Forum | 2 | 01-08-2008 04:15 AM |