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06-19-2007, 03:39 PM
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 Tips on Primer Dilution and Concentration for PCR hello all, I have some old and long methods to get primer dilution factors for the right concentration for PCR. Does anyone have some quick and new methods (ie website program) to do this? thanks!         |
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08-03-2007, 12:12 PM
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 Re: Tips on Primer Dilution and Concentration for PCR when u have lyophilized primer (dried form), add TE buffer (pH 7) to make concentration 100 µM. Then dilute it to make 10µM working solution. This stock can directly be used for PCR. If u use 1µl of this stock in PCR mix (25µl) u are using 0.4 µM of primer in final mix. Hope i have solved ur problem. |
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