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06-19-2007, 02:35 PM
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 Colony PCR Problems Hello, I am conducting colony PCR however I get a problem as I do not get any positive results from the experiment! Does anybody have a good protocol for E.coli colony PCR? My protocol is: Pick colony using pipette Put the pricked colony in 20 ul of milliQ Water. Heat sample for 8 min (boil) Use 1 ul of this mix as PCR template and conduct PCR. Should I use something like an enzyme to extract DNA? Thanks        |
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06-29-2007, 02:29 PM
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 Re: Colony PCR Problems when i do colony pcr i pick a colony (small-ish) with an sterile tip and add it directly to the pcr mix... the high temps during pcr will lyse your bugs... it had always worked for me |
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