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PCR - Polymerase Chain Reaction Forum


Positive results then negative results on DNA extracted from mosquito

PCR - Polymerase Chain Reaction Forum

PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.



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Old 06-14-2007, 07:03 AM
Pipette Filler
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Default Positive results then negative results on DNA extracted from mosquito

Does mosquito DNA have any known PCR inhibitors? We have run DNA extracted from mosquitoes. Came out positive the first time and then negative. Several samples have come out like this. What do you do to overcome this inhibition?
Please need your help!

Thanks,
Mt.

Last edited by Mtawa; 06-14-2007 at 07:09 AM.
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