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06-13-2007, 09:33 AM
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 Any tips for amplifying small amplicons?? Hi everyone! I am trying to optimize my PCR reaction for a 120bp amplicon. In my master mix i am using dmso at 5% to decrease the primer dimer formation.Shall I decrease that or shall I play with the MgCl2 concentration? Any suggestions?        |
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07-23-2007, 03:38 PM
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| Re: Any tips for amplifying small amplicons?? I have never found the need to use DMSO, much less optimize it. I solve primer dimer formation by optimizing for primer-concentration first, annealing temperature and MgCl2 second, third I would optimize for starting DNA concentration. |
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