I'm currently looking at some microsatellites in a species of sunflower. Initially, we extracted DNA from seeds, which has worked nicely. Getting suitable DNA from leaf tissue has been more difficult. I'm guessing that there's secondary compounds in the leaf tissue that are interferring with the pcr reaction.
We recently ran some samples of DNA derived from leaves using different extaction protocols. Some of these were successful in getting microsat amplification. However, we observed something odd, and I was wondering if anyone might have a possible explanation.
In the reactions that resulted in amplified microsats we also got primer dimers. In reactions that did not result in amplified microsats we got no primer dimers. I'm puzzled. What could be going on here?