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Long PCR - how to setup and optimize?

Long PCR - how to setup and optimize? - PCR - Polymerase Chain Reaction Forum

Long PCR - how to setup and optimize? - PCR - Polymerase Chain Reaction Forum. Discuss and ask questions about PCR troubleshooting, PCR protocols and methods, PCR products, and PCR theory.


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Old 07-03-2006, 07:14 PM
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Question Long PCR - how to setup and optimize?



How can I setup long pcr?

i want to PCR regions up to 15 kb accurately. Does anyone have experience with this?

thank you
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Old 01-19-2010, 01:25 PM
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Default Re: Long PCR - how to setup and optimize?

Hi! I am looking for the same info. Will get back if I come across anything.

There are special enzymes for this purpose.
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Old 01-25-2010, 07:45 PM
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Default Re: Long PCR - how to setup and optimize?

Try Expand Long Template PCR system from Roche
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Old 03-29-2010, 07:33 AM
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Default Re: Long PCR - how to setup and optimize?

KOD-FX from Toyobo or LA-Taq from Takara are also good.
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