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| Urgent help needed.... I am trying to amplify a gene from strep chormosomal DNA extraction to clone. Works beautifully with TAQ but when i try the same conditions using pfu hot start doesn't work at all.............Have tried manufacturers instructions..doesn't work!! getting desperate...Please trouble shoot... Strep girl ![]() |
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| i am really sorry to disturb you but i would like to tell you that right now i am working on an amplification of a gene with pfu and unfortunately it does not work so i was wondering what have you done and if you have resolved the problem. please let me know as soon as possible because these experiments are really important for my university thesis |
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