Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Chemistry Forum > Organic Chemistry Forum
Register Search Today's Posts Mark Forums Read

Organic Chemistry Forum Organic Chemistry Forum


Sephadex LH-20 Replacement-Question

Sephadex LH-20 Replacement-Question - Organic Chemistry Forum

Sephadex LH-20 Replacement-Question - Organic Chemistry Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-25-2010, 07:53 AM
Pipette Filler
Points: 12, Level: 1 Points: 12, Level: 1 Points: 12, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Sephadex LH-20 Replacement-Question



To whom it may concern,

I am synthesizing some dyes according to this patent: "Sulfonated xanthene derivatives" Patent#: 6130101
There are some cleaning steps with Sephadex LH-20 in it e.g.
[...]
Example 8
Preparation of Compound 1: ##STR25##
5-(and-6)-Carboxyrhodamine 110, hydrochloride (8.14 g, 19.8 mmol; Molecular Probes, Inc., Eugene, Oreg.) is slowly added in portions to 30% fuming H 2 SO 4 (50 mL) in an ice bath. After 12 hrs. at 0 C., the solution is poured into 600 mL cold dioxane, and 1.2 L Et 2 O is added. The suspension is filtered through diatomaceous earth. The filter cake is suspended in 1.2 L MeOH and the pH is adjusted to .about.10 with triethylamine. The mixture is filtered and the filtrate is evaporated. The residue is purified on SEPHADEX LH-20 using water as the eluant to give Compound 1 as an orange solid (9 g).
[...]

Is there a way to replace the Sephadex LH-20 with a cheaper media or a other method of cleaning?

Thank you for the help

Mahalo

Udo
Reply With Quote
  #2  
Old 08-15-2010, 09:34 PM
Pipette Filler
Points: 13, Level: 1 Points: 13, Level: 1 Points: 13, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2010
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Sephadex LH-20 Replacement-Question

hi everyone
Reply With Quote
  #3  
Old 08-16-2010, 05:34 AM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2010
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Sephadex LH-20 Replacement-Question

hi my name is walter..
Reply With Quote
  #4  
Old 02-12-2013, 02:03 PM
Summer Student
Points: 240, Level: 4 Points: 240, Level: 4 Points: 240, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 56
Thanks: 0
Thanked 0 Times in 0 Posts
Default ジョーダン 11

[

[

[
Reply With Quote
  #5  
Old 02-12-2013, 02:48 PM
Summer Student
Points: 240, Level: 4 Points: 240, Level: 4 Points: 240, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 56
Thanks: 0
Thanked 0 Times in 0 Posts
Default ジョーダン 11

[

[

[
Reply With Quote
  #6  
Old 02-12-2013, 03:52 PM
Summer Student
Points: 240, Level: 4 Points: 240, Level: 4 Points: 240, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2013
Posts: 56
Thanks: 0
Thanked 0 Times in 0 Posts
Default エアジョーダン 2013

[

[

[
Reply With Quote
Reply

Tags
lh20 , replacementquestion , sephadex


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
question about phenol/water saturated solution Z.L. K Protocols and Methods Forum 2 02-11-2008 01:05 AM
Sci.chem FAQ - Part 1 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:06 AM


All times are GMT. The time now is 12:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14247 seconds with 16 queries