Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Omics and Genomics Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

Omics and Genomics Forum Discuss and post questions about Genomics, Proteomics, Drug discovery, and Interactome research.


RNAase solution

Omics and Genomics Forum

Discuss and post questions about Genomics, Proteomics, Drug discovery, and Interactome research.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 02-12-2007, 04:25 PM
M.D/Ph.D
Points: 5,456, Level: 50Points: 5,456, Level: 50Points: 5,456, Level: 50
Activity: 81%Activity: 81%Activity: 81%
 

Join Date: Jun 2006
Location: Pakistan
Posts: 424
aftabac RSS Feed
Send a message via MSN to aftabac Send a message via Yahoo to aftabac
Default RNAase solution

Hi
i want to prepare RNAase solution, so for this whether i should dissolve the RNAase enzyme in distilled water or i should put it in some buffer, if some buffer is required then what is best buffer.



regards
aftab
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

  #2 (permalink)  
Old 02-13-2007, 09:02 AM
kiki06's Avatar
Post-Doc
Points: 3,937, Level: 41Points: 3,937, Level: 41Points: 3,937, Level: 41
Activity: 0%Activity: 0%Activity: 0%
 
Join Date: Feb 2006
Posts: 257
kiki06 RSS Feed
Default Re: RNAase solution

Dear Aftab!

how are you? We missed you at the forum!

How are your studies?

As to your question, I have used many RNAses. Most come already in buffer solution. I have used RNaseT1, an RNAse mix solution and RNase A.

All come ready in buffer and can be added to solutions. They are quite strong enzymes and will quickly digest RNA at 37 C especially if you add them by the unit (U).

If you need more information just post here,
Kiki
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #3 (permalink)  
Old 07-19-2007, 04:57 PM
danfive's Avatar
Nobel Laureate
Points: 2,377, Level: 31Points: 2,377, Level: 31Points: 2,377, Level: 31
Activity: 18%Activity: 18%Activity: 18%
 

Join Date: Jul 2007
Location: Houston TX
Posts: 509
danfive RSS Feed
Default Re: RNAase solution

Dilute it in RNase solution not water. PCR buffer or similar can be acceptable, remember the rnase is a protein and to maintain structure and activity will need an appropriately buffered solution.
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Anyone knows how to precipitate RNA from 100% formamide deionzes solution? piaojian RNA Techniques Forum 1 02-01-2008 07:46 PM
How to Prepare 100% TCA Solution? dave2006 Basic Lab Protocols and Techniques 0 01-04-2008 02:33 AM
Using HCl for Proteins i Aqueous solution Apopov Protein Science 2 12-04-2007 08:23 PM
solution to growing of LNCaP geminisc Cell Biology and Cell Culture 0 07-20-2007 01:11 PM
How should I make LB solution? Dionisia Basic Lab Protocols and Techniques 1 02-02-2006 02:20 AM


All times are GMT. The time now is 09:40 PM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved