RNAase solution

[aftabac]

Hi
i want to prepare RNAase solution, so for this whether i should dissolve the RNAase enzyme in distilled water or i should put it in some buffer, if some buffer is required then what is best buffer.



regards
aftab

[kiki06]

Dear Aftab!

how are you? We missed you at the forum!

How are your studies?

As to your question, I have used many RNAses. Most come already in buffer solution. I have used RNaseT1, an RNAse mix solution and RNase A.

All come ready in buffer and can be added to solutions. They are quite strong enzymes and will quickly digest RNA at 37 C especially if you add them by the unit (U).

If you need more information just post here,
Kiki

[danfive]

Dilute it in RNase solution not water. PCR buffer or similar can be acceptable, remember the rnase is a protein and to maintain structure and activity will need an appropriately buffered solution.