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plasmid isolation

plasmid isolation - New Member Introductions Forum

plasmid isolation - New Member Welcome & Introductions Forum. Please introduce yourself here as a new member, let us know a bit more about yourself!


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Old 07-29-2013, 10:20 AM
Pipette Filler
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Default plasmid isolation



i have read your query about pcr mutagenesis.
Even i am facing the same problem in Site directed mutagenesis.
After getting colonies, I am not able to isolate plasmid from XL1-Blue transformed supercompetent cells and even not able to transform it further in DH5a E.coli cells.
Please suggest me as i have stucked in between and not able to give samples for sequencing.
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Old 07-29-2013, 10:27 AM
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Default welcome to molecular station

Hi jayesh desale! welcome to the Molecular Station! glad to have you join us
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Old 07-29-2013, 10:27 AM
Pipette Filler
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Hi jayesh desale! welcome to the Molecular Station! happy to have you here
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