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New to RNA

New to RNA - New Member Introductions Forum

New to RNA - New Member Welcome & Introductions Forum. Please introduce yourself here as a new member, let us know a bit more about yourself!


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Old 08-01-2012, 01:05 PM
Pipette Filler
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Join Date: Aug 2012
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Default New to RNA



Well we work are our life on one medium (proteins) and the next day, we change job and introduce to the wonderful world of RNA.
At less not all the work before is down the . I work on small nuclear RNA in gene splicing.
I try the Mirvana kit to isolate them and got and low yield of 8 to 13 ug in 100 ul out of 5 million cells.
Not enough for sequencing or even to control the quality of the RNA purified

Is it the best way to do it or there is something else out there
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Old 08-01-2012, 02:33 PM
oBWhat's Avatar
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Welcome to Molecular Station. Enjoy your stay here...
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Old 08-01-2012, 02:33 PM
Pipette Filler
 
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Default welcome

Hi boum13! welcome to the Molecular Station! glad to have you join us
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