Our lab is a hematology lab. Today we found out with the help of microbiology lab that our cultures were infected with Pseudomonas aeruginosa
and Stenotrophomonas maltophilia
. We work with bone marrow derived human MSCs, and as u understand it is difficult to retrieve any sample lost... So we think we should complete our medium with ciprofloxacin a fluoroquinol analogue which perturbs the bacterial DNA gyrase and mamalian topoisomerase II. What concentration should we use? Is there any better antibiotic we could use, which would have less adverse effects on our cells?