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#1
03-22-2012, 01:34 PM
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 Annealing Temperature Kindly note that I have three genes with the below listed Tm and I would like to use RT-PCR SYBR Green application method. What is the best Annealing Temperature may I have to use to get good results. ? Primer F Gene 1 Tm: 53oC Primer R Gene 1 Tm: 54oC Primer F Gene 2 Tm: 53oC Primer R Gene 2 Tm: 52oC Primer F Gene 3 Tm: 55oC (GAPDH) Primer R Gene 3 Tm: 49oC (GAPDH) Thanks Masood  |
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