Molecular Research Topics Forum

Real-Time PCR and Quantitative PCR Forum

Post Questions and Discuss Biostatistics Programs and Stastistical Analysis Questions

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.

EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.

Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.

Ask Questions and Discuss BioPhysics. Talk about BioPhysics Protocols and Techniques. BioPhysics Forum

Discuss gene therapy issues, methods and protocols for viral gene therapy, problems and solutions.

Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.

Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.

Transfection Forum. Cell transfection discussion including stable cells transfection, transient transfection. Troubleshooting and posting of discussions on lipofection, and reagents: Fugene, lipofectamine, and others.

All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques

Signalling is an integral part of the cell neural network. Phosphorylation and other protein modifications modulate this interaction. Post and discuss the study of protein-protein interactions, and signalling biology protocols and science here.

Stem Cells and Stem Cell Culture. Embryonic Cell Forum and Mouse Transgenic Forum. Blastocyst injection, ES Cell Selection.

SDS-PAGE Forum and Gel Electrophoresis Forum. Discuss the running of agarose gels, sds-page gels, and other gels including sequencing, gradient or tricine.

DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.

cDNA Forum. Discuss complementary DNA synthesis, cDNA libraries, and cDNA cloning in this forum.

Chromatin Immunoprecipitation Forum. Discuss, chat about, and post questions about the ChIP assay, in the ChIP forum. Includes troubleshooting, protocols, buffer preparation, and more.

Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.

Sub-Forums: Immunoprecipitation Forum

Discuss western blotting and immunoblot in the western blot forum discussion board. Ask Questions about transfers, blocking, membrane antibody incubation and exposures.

Discuss research on protein crystallography and crystalization. Protein Crystallography Forum.

Recombinant Protein Forum. Discuss purification, expression, and isolation of recombinant and cloned proteins here.

ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.

Protein Forum

Peptide Forum. Ask and discuss questions on peptide protocols, custom peptide synthesis, peptide identification and peptide sequencing.

Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.

2-D Gel Electrophoresis Forum. A forum dedicated to two dimensional gel electrophoresis, discussion about systems, and troubleshooting its problems.

Microarray Forum. Discuss array technology and Gene Chips. DNA microarray and Protein arrayit forums.

Post Genomics Questions and Discuss Genomics Here.

Discuss and Post Questions in this forum about RNA interference and siRNA. RNAi and siRNA transfection, inhibition of endogenous genes, and miRNA and shRNA.

Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.

Immunocytochemistry Forum. Discuss methods and immunocytochemistry techniques for cell suspension, cytospin, or cultured cells.

Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.

Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.

Discuss research on yeast the model organism. Post questions and topics on the molecular biology and genetics of Saccharomyces cerevisiae and other yeast species.

Zebrafish Danio Rerio Forum. Discuss zebrafish, aquarium culture and molecular biology lab specimens.

Botany Forum

C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.

Discuss Arabidopsis and Plant Biology Research.

Discuss Microbiology Science and Protocols here. Post questions on the study of viruses, fungi, parasites and bacteria here. Microbiology Forum.

Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.