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Cloning unknown promotor

Cloning unknown promotor - Molecular Cloning Forum

Cloning unknown promotor - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 04-07-2014, 09:27 AM
Pipette Filler
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Default Cloning unknown promotor



Dear all,

I want to clone a promotor and test it with a GFP fusion construct in vivo later on. I have looked at the cDNA sequence as listed on Ensembl. The startcodon of the gene of interest lies within the second exon. The 5' UTR is about 500 bp long. I want to start with an amplificaton of 3 kb and test that piece of sequence. I am unsure about the 5 'UTR, do I have to include that or not?

Thank you.
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Old Yesterday, 07:40 AM
Pipette Filler
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Default Re: Cloning unknown promotor

blast !
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