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fushi tarazu 04-07-2014 09:27 AM

Cloning unknown promotor
 
Dear all,

I want to clone a promotor and test it with a GFP fusion construct in vivo later on. I have looked at the cDNA sequence as listed on Ensembl. The startcodon of the gene of interest lies within the second exon. The 5' UTR is about 500 bp long. I want to start with an amplificaton of 3 kb and test that piece of sequence. I am unsure about the 5 'UTR, do I have to include that or not?

Thank you.

lzhang6060 09-16-2014 07:40 AM

Re: Cloning unknown promotor
 
blast !


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