Cloning unknown promotor
I want to clone a promotor and test it with a GFP fusion construct in vivo later on. I have looked at the cDNA sequence as listed on Ensembl. The startcodon of the gene of interest lies within the second exon. The 5' UTR is about 500 bp long. I want to start with an amplificaton of 3 kb and test that piece of sequence. I am unsure about the 5 'UTR, do I have to include that or not?
Re: Cloning unknown promotor
|All times are GMT. The time now is 11:04 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved