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Yeast pAG25 vector_NatMX promoter

Yeast pAG25 vector_NatMX promoter - Molecular Cloning Forum

Yeast pAG25 vector_NatMX promoter - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 03-05-2013, 03:14 PM
Pipette Filler
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Default Yeast pAG25 vector_NatMX promoter



Hi,

I was wondering under which promoter is the NatMX cassette expressed in the pAG25 plasmid. I need to clone a sequence upstream of the Nat1 gene on the vector and don't want to disrupt the promoter. But I don't know where it is or what is its sequence...

Thanks for your help.
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