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pgl3 promoter vector

pgl3 promoter vector - Molecular Cloning Forum

pgl3 promoter vector - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 12-12-2012, 05:38 PM
Pipette Filler
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Default pgl3 promoter vector



Hello everybody!

I have the pGL3 promoter vector from Promega and I have cloned the different alternative transcripts of the gene I am studying. I would like to observe differences in the transcriptional regulation of the transcripts as a result of luciferase activity. Promega recommends three different luciferase assay systems, steady-glo, bright-glo and one-glo with this vector. Does anyone have any recommendation regarding these three systems in order to obtain differences in transcription?

Thanks in advance!
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alternative transcripts , luciferase , pgl3 , promoter , vector


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