| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-28-2012, 09:35 PM
| |||||||||||
| |||||||||||
 one question about TA cloning hi all， I have one question about TA cloing. My insert has restrict enzyme sites at both end (5' end BamH I and 3' end Xhol I), If my insert is in the reverse orientation, can it still be digested ? thanks in advance  |
|
#2
11-30-2012, 05:59 AM
| |||||||||||
| |||||||||||
 Re: one question about TA cloning Hi, Even tough the insert ligated in reverse orientation you will be able to digest with the mentioned enzymes. What ever is the orientation, your insert is between the two restriction sites know. If these mentioned enzymes are cutting in between the insert , then you have to calculate the expected size fragments considering both the orientations. Based on the result you can judge the orientation of insert. |
|
| Tags |
| cloning , question |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Basic cloning question | weenold | Molecular Cloning Forum | 1 | 08-13-2011 01:37 PM |
| Cloining Survey for School Assignment | angelicsnow | Biology Forum | 0 | 08-29-2009 06:44 AM |
| help on difficult cloning | Senkovich, Olga | Protocols and Methods Forum | 1 | 05-05-2007 12:14 AM |
| BioInfoMan:reduce your working time on molecular cloning by 80% | gaxl | Bioinformatics | 2 | 08-15-2006 12:31 AM |
| basic cloning question | Sarah LaMere | Protocols and Methods Forum | 2 | 07-29-2005 09:56 PM |
Linear Mode
