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| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
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#1
09-12-2012, 04:35 AM
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 How to insert Transcriptional Terminator Sequence in vector Hi, I am planning on cloning 2 genes in a pet vector. I already have a construct in which one gene is cloned downstream of the promoter, now in order to insert next gene of interest in the same vector I will have to incorporate Shine' Dalgarno sequence in the 5' primer of the 2nd gene, how can I incorporate Transcriptional Terminator Sequence at the end of 2nd Gene? Thanks!  |
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| insert , sequence , terminator , transcriptional , vector |
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