Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Register Search Today's Posts Mark Forums Read

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


How to insert Transcriptional Terminator Sequence in vector

How to insert Transcriptional Terminator Sequence in vector - Molecular Cloning Forum

How to insert Transcriptional Terminator Sequence in vector - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-12-2012, 04:35 AM
Volunteer
Points: 2,091, Level: 28 Points: 2,091, Level: 28 Points: 2,091, Level: 28
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2010
Posts: 31
Thanks: 1
Thanked 0 Times in 0 Posts
Default How to insert Transcriptional Terminator Sequence in vector



Hi,

I am planning on cloning 2 genes in a pet vector. I already have a construct in which one gene is cloned downstream of the promoter, now in order to insert next gene of interest in the same vector I will have to incorporate Shine' Dalgarno sequence in the 5' primer of the 2nd gene, how can I incorporate Transcriptional Terminator Sequence at the end of 2nd Gene?

Thanks!
Reply With Quote
Reply

Tags
insert , sequence , terminator , transcriptional , vector


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Plasmid-stability? Fragment-deletion? I'm in dire need to identify and solve problems TGS Molecular Cloning Forum 3 10-22-2012 09:17 PM
Very high insert to vector ratio during ligation. jerseysurfer66 Molecular Cloning Forum 0 03-25-2009 07:38 PM
insert with blunt ends + vector with sticky ends? does it work? trypsin Molecular Cloning Forum 7 08-31-2008 09:11 AM
DNA Cloning Protocol moleculardude Molecular Biology Articles and Protocols 2 08-05-2007 02:31 PM
Ligation Deitiker, Philip R Protocols and Methods Forum 0 06-21-2007 09:12 PM


All times are GMT. The time now is 05:16 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11385 seconds with 16 queries