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-   -   How to insert Transcriptional Terminator Sequence in vector (http://www.molecularstation.com/forum/molecular-cloning-forum/86419-how-insert-transcriptional-terminator-sequence-vector.html)

rasing02 09-12-2012 04:35 AM

How to insert Transcriptional Terminator Sequence in vector
 
Hi,

I am planning on cloning 2 genes in a pet vector. I already have a construct in which one gene is cloned downstream of the promoter, now in order to insert next gene of interest in the same vector I will have to incorporate Shine' Dalgarno sequence in the 5' primer of the 2nd gene, how can I incorporate Transcriptional Terminator Sequence at the end of 2nd Gene?

Thanks!


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