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|Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.|
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pgem by promega or TOPO by invitrogen
I would like to clone plasmid vectors using either TOPO or pGEM. After i clone the vectors I would like to use Riboprobe by promega to make probes for in situ. I read an article that compared the two based on base pairs.
This showed that TOPO worked better for products under 1kB. My PCR products are under 1kb so I would like to use TOPO, but is there an issue with using invitogens TOPO and promegas Riboprobe?
|invitrogen , pgem , promega , topo|
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