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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


pgem by promega or TOPO by invitrogen

pgem by promega or TOPO by invitrogen - Molecular Cloning Forum

pgem by promega or TOPO by invitrogen - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 08-22-2012, 08:03 PM
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Default pgem by promega or TOPO by invitrogen



I would like to clone plasmid vectors using either TOPO or pGEM. After i clone the vectors I would like to use Riboprobe by promega to make probes for in situ. I read an article that compared the two based on base pairs.

This showed that TOPO worked better for products under 1kB. My PCR products are under 1kb so I would like to use TOPO, but is there an issue with using invitogens TOPO and promegas Riboprobe?
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