Our lab would like to insert our pcr products into a plasmid, insert the plasmid into a bacteria, store it, then preform a reverse transcription on the inserted gene so that we can use the rna produced from the product as a probe for in situ.
1. Is this a good way to create your own probe?
2. If so what supplies will be need? We have everything to do pcr and gel extractions and that is it.
3. What literature/protocols are there on this topic so that I can become familiar with the best way to do this.
Thank you so much for your help.