I've never designed or worked with NKK degenrate priemrs before, so I'm a bit lost.
(Admittedly, I'm not the most experienced person when it comes to primer design or cloning, so please forgive any stupid questions).
I want to design an NKK degenerate primer for creating a mutagenesis library for evolving an enzyme. However, I cannot really figure out how to desgin them. The software available ususally focuses on designing these primers using sequence alignments or using them for sequencing purposes.
Does anyone have experience with this designing type of PCR/ primers?