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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


TOPO XL vector

TOPO XL vector - Molecular Cloning Forum

TOPO XL vector - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 01-25-2012, 11:09 AM
Pipette Filler
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Default TOPO XL vector



Hi everybody!
Has anyone cloned long fragments into the TOPO XL vector? In the manual there is not mention of the amount of starting PCR product to use in the TOPO cloning reaction, but only the ratio of 4ul PCR product + 1ul pCR XL TOPO vector.
Has anyone experience with this or any suggestion?
Thank you very much
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