Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Register Search Today's Posts Mark Forums Read

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Where can I find expire date for Topo cloning kit

Where can I find expire date for Topo cloning kit - Molecular Cloning Forum

Where can I find expire date for Topo cloning kit - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-01-2011, 03:30 PM
Pipette Filler
Points: 523, Level: 10 Points: 523, Level: 10 Points: 523, Level: 10
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Where can I find expire date for Topo cloning kit



I have a topo clonging kit which was opened on 6/10/11. I used it for cloning and few clones. I am trying to figure out what is problem. Is it possibe the kit already expired? but I can not find the expire date from the kit. How much the old kit will affect the cloning? Thanks for reply.
Reply With Quote
  #2  
Old 12-01-2011, 06:43 PM
Graduate Student
Points: 1,231, Level: 20 Points: 1,231, Level: 20 Points: 1,231, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 113
Thanks: 0
Thanked 16 Times in 13 Posts
Default Re: Where can I find expire date for Topo cloning kit

Quote:
Originally Posted by lily5658 View Post
I have a topo clonging kit which was opened on 6/10/11. I used it for cloning and few clones. I am trying to figure out what is problem. Is it possibe the kit already expired? but I can not find the expire date from the kit. How much the old kit will affect the cloning? Thanks for reply.

I think if the kit was stored at the correct temperature it will probably be fine. I have used enzymes that "expired" 10 years ago and they work just fine as long as they are kept at -20C.

Do a test experiment with a known positive control, if that doesn't work then you know the kit is off.
Reply With Quote
Reply

Tags
cloning , date , expire , find , kit , topo


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How to find a hot date in 24 hours using sleep programming rosabellapetit@yahoo.com Physics Forum 0 08-20-2008 03:47 PM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
The Theory of Moving Dimensions: The Time Dimension is Moving Relative to The Three Spatial Dimensions Captain Ranger McCoy Physics Forum 14 07-03-2005 03:14 AM
Sci.chem FAQ - Part 1 of 7 Bruce Hamilton Chemistry Forum 0 01-15-2004 09:06 AM


All times are GMT. The time now is 03:39 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14416 seconds with 16 queries