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| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
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#1
12-01-2011, 02:30 PM
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 Where can I find expire date for Topo cloning kit I have a topo clonging kit which was opened on 6/10/11. I used it for cloning and few clones. I am trying to figure out what is problem. Is it possibe the kit already expired? but I can not find the expire date from the kit. How much the old kit will affect the cloning? Thanks for reply.  |
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#2
12-01-2011, 05:43 PM
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| Re: Where can I find expire date for Topo cloning kit Quote:
I think if the kit was stored at the correct temperature it will probably be fine. I have used enzymes that "expired" 10 years ago and they work just fine as long as they are kept at -20C. Do a test experiment with a known positive control, if that doesn't work then you know the kit is off. |
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| cloning , date , expire , find , kit , topo |
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