Hello everyone My question is about a problem I'm having finishng the sequence of a gene. I'm using the Genome Walking Kit from Clontech for this process, the PCR products from genome walking are inserted into the vector pCRII and sequenced'm with the primers M13F and M13R from the vector. The problem is that in the results of the sequencing I can not find the known sequence of my gene segment (from where the reverse primers were designed), but I find the adapter kit GW (the forward primer is complementary to this adaptor which is ligated to digested genomic DNA before the PCR reaction). In the secuence I have both ends of the vector, which discard the possibility that the segment inserted is not completely read. If contamination would not have the adapter sequence of the kit. Anyone know anything about this? Thanks!!!