I'd like to clone a gene + promoter into pBR322 and express it in a strain (not for overexpression purposes). I have cloned into plasmids before to overexpress genes and then it's obvious where to put the genes i.e. MCS. However, I'm a bit confused as to whether the gene will be expressed properly if I just whack them in the middle of the Amp or Tet gene. Do I need to encode a stop codon upstream of my promoter to prevent read-through. Does it need to be in the same orientation as the gene I'm cloning it into?