Hey everyone! I hope I'm posting this in the correct forum!
I isolated the internal transcribed spacer region of a fern leaf and transformed into E. coli using a pCR4 TOPO vector. I isolated the PCR product again using EcoRI. My professor then prepared it and sent it off for sequencing.
When the sequences came back, I trimmed off the vector sequence still attached and ran a BLAST search to verify that I had fern. But when I ran the search, the sequence came back saying that it belonged to SPINACH!!! So surprising!
I have to write a lab report over this and I have no idea how fern turned into spinach. I read an article talking about contamination, but I have no idea how fern would come into contact with spinach. It was definitely a fern plant, and not spinach. Any ideas are welcomed! Thanks