Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Register Search Today's Posts Mark Forums Read

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Recombinants and fusion protein

Recombinants and fusion protein - Molecular Cloning Forum

Recombinants and fusion protein - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-16-2011, 05:59 AM
Pipette Filler
Points: 56, Level: 1 Points: 56, Level: 1 Points: 56, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Recombinants and fusion protein



Hello, I am looking for some guidance or help with my homework for the Molecular Biology Laboratory. Below are the questions. I don't really understand how to troubleshoot for these things.

Thank you very much for your help!!

By the way, the vector that we use in the lab is pGLO( bgl2) and the inserts are-Actin 3, Actin 8, and Actin 12.



1. Explain how a sample could show a successful ligaton between a vector ( which contains a green fluorescence protein-GFP ) and an insert ( via colony PCR and a band larger from the ligation colony than the vector alone) but not show green GFP protein expression.

2. Explain how a sample could show a successful ligation between a vector( which contains a green fluorescence protein-GFP ) and an insert ( via colony PCR and a band larger from the ligation colony than the vector alone), but not be a fusion protein. In other words, the GFP from the recombinant detected on the Western blot is the same size as the GFP protein from the control vector plasmid.
Reply With Quote
Reply

Tags
biotechnology , fusion , gfp fusion protein , molecular biology , protein , recombinants , troubleshooting


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 10:27 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10899 seconds with 15 queries