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Transcription termination sequences

Transcription termination sequences - Molecular Cloning Forum

Transcription termination sequences - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 03-02-2011, 04:06 PM
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Default Transcription termination sequences



Hi - I'm new at this so forgive the dumb questions please but if I have a Gateway enabled vector in which I am inserting my favourite promoter and GFP tag (with stop codon) in order to get a transcriptional reporter, do I still need a termination sequence downstream of the Gateway casette for proper expression once transformed into a plant (not using agro!) ?

The vector has one, but when the gateway casette was inserted by the person we got it from, the terminator sequence is now out of frame.....
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