In a mixture of complimentary primer and vector, will agarose gel electrophoresis separate them if they have annealed but not ligated? Also in a transformation of unligated vector can bacteria take up primer annealed but not ligated to a complimentary vector?
My reason for asking is I have primers designed for regular mutagenesis which I am now using with a different system. These primers are overlapping and produce sticky ended vectors, for various reasons this system you are supposed to produce blunt ended. However I'm on a very tight budget and would like to save myself the expense of reordering these primers if possible.