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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Viral Growth Conditions

Viral Growth Conditions - Molecular Cloning Forum

Viral Growth Conditions - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 02-20-2011, 06:22 PM
Pipette Filler
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Default Viral Growth Conditions



Hi everyone,

I have a viral sequence in a pcDNA3.1- backbone. I have a couple of questions.

1) I was wondering if the transformation conditions are particular to pcDNA3.1-, i.e. at 37C, or if I have to grow them as a virus?


2) I've been told that to viral growth conditions are the following: heat shock the cells at 37C, then transform at 30C and grow the cells in LB agar (with the appropriate antibiotic) at 30C. Does this seem right for viral growth conditions?

Thank you so much for your time and help!

Maria
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