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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


No Right Insert During Cloning!

No Right Insert During Cloning! - Molecular Cloning Forum

No Right Insert During Cloning! - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 02-18-2011, 08:47 PM
Pipette Filler
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Default No Right Insert During Cloning!



I am trying to clone a 1,200 bp PCR products of PISTILLATA in the plants. I did directly clone the PCR products. The half of the clones was white. When I check the size of the insert by PCR amplification, there was no right insert (50-100 bs of inserts). I ran the PCR products on the gel, and cleaned the right fragment from the gel. This time, the cloning did not work at all. Only 2-4 blue clones, nothing more on the plate. I did this test several times, but no success. Anybody can suggest any solution? Thank you.

Last edited by admin; 02-19-2011 at 12:28 AM.
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