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No Right Insert During Cloning!
I am trying to clone a 1,200 bp PCR products of PISTILLATA in the plants. I did directly clone the PCR products. The half of the clones was white. When I check the size of the insert by PCR amplification, there was no right insert (50-100 bs of inserts). I ran the PCR products on the gel, and cleaned the right fragment from the gel. This time, the cloning did not work at all. Only 2-4 blue clones, nothing more on the plate. I did this test several times, but no success. Anybody can suggest any solution? Thank you.
Last edited by admin; 02-18-2011 at 11:28 PM.
|cloning , insert|
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