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| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
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#1
02-16-2011, 06:19 PM
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 Cloning two CDS with different reading frames Hello, I'm starting a cloning proyect in which I need to clone a gene that has a +2 reading frame and the reporter gene has a +1 frame. I'm trying to do a c-ter fusion and I don't know if there will be any problem having these coding sequences with different reading frames. What can I do to change this? Thanks for your help, Talin  |
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| cds , cloning , frames , reading |
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