| | Re: double transformation in e.coli
There are strains of E. coli harbouring the pLysS plasmid that are further transformable. This competent cells are comercial, so you can get your own from many manufacturers.
If you transform this competent cells, you will have a clone that's resistant to Cloramphenicol and another antibiotic.
If you're asking whether or not you can transform with two vectors at the same time, I think it'd be hard, but not impossible. I've not done it myself.
Last edited by SebaQ; 01-11-2011 at 03:23 PM.