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PET vectors pGEX 2T and pGEX 3X

PET vectors pGEX 2T and pGEX 3X - Molecular Cloning Forum

PET vectors pGEX 2T and pGEX 3X - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 11-19-2010, 05:27 AM
Pipette Filler
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Default PET vectors pGEX 2T and pGEX 3X



Hello. does anybody know the difference between pGEX 2T and pGX 3x expression vector(PET) reading frames. it says pGX 3X is out of frame and pGX 2T is in frame.i do not know what does it actually mean
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