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Cloning large insert in pQE30,expressing it!
I like to fuse two genes,one of 1.6Kb and another of 1.7Kb in pQE30 vector of 3.4Kb.After that I want express the fused protein.Is it possible to clone 3.7Kb insert into pQE30(3.4 Kb) and its expression (fusion protein) is possible?
Already i cloned 1.6Kb insert into pQE30 and expressed the protein and got the result.
Is there any other expression vector where i can clone my fused 3.7Kb gene?
Give me suggestion plz,most useful to me......
Re: Cloning large insert in pQE30,expressing it!
Sorry for the delay ^^.
I've succesfully cloned a 4.1 gene into a 3 Kb expression vector and got results, so it is possible to do it. And for expression vectors you can use, try looking at this:
[Only registered users see links. ]
|cloning , expressing , insert , large , pqe30|
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