Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Register Search Today's Posts Mark Forums Read

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Cloning large insert in pQE30,expressing it!

Cloning large insert in pQE30,expressing it! - Molecular Cloning Forum

Cloning large insert in pQE30,expressing it! - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-24-2010, 11:21 AM
Pipette Filler
Points: 1,078, Level: 18 Points: 1,078, Level: 18 Points: 1,078, Level: 18
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2009
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Cloning large insert in pQE30,expressing it!



Hi,
I like to fuse two genes,one of 1.6Kb and another of 1.7Kb in pQE30 vector of 3.4Kb.After that I want express the fused protein.Is it possible to clone 3.7Kb insert into pQE30(3.4 Kb) and its expression (fusion protein) is possible?

Already i cloned 1.6Kb insert into pQE30 and expressed the protein and got the result.

Is there any other expression vector where i can clone my fused 3.7Kb gene?

Give me suggestion plz,most useful to me......
Thanks
Regards,
Chitra.
Reply With Quote
  #2  
Old 06-10-2010, 06:24 PM
Pipette Filler
Points: 1,078, Level: 18 Points: 1,078, Level: 18 Points: 1,078, Level: 18
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2009
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Cloning large insert in pQE30,expressing it!

no reply still now??????????
Reply With Quote
  #3  
Old 06-10-2010, 09:38 PM
Graduate Student
Points: 2,626, Level: 33 Points: 2,626, Level: 33 Points: 2,626, Level: 33
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Location: Santiago, Chile
Posts: 100
Thanks: 1
Thanked 24 Times in 24 Posts
Default Re: Cloning large insert in pQE30,expressing it!

Sorry for the delay ^^.

I've succesfully cloned a 4.1 gene into a 3 Kb expression vector and got results, so it is possible to do it. And for expression vectors you can use, try looking at this:

[Only registered users see links. ]
Reply With Quote
Reply

Tags
cloning , expressing , insert , large , pqe30


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
DNA Cloning Protocol moleculardude Molecular Biology Articles and Protocols 2 08-05-2007 02:31 PM
GATEWAY cloning of large PCR fragments Ricky Boernke Protocols and Methods Forum 1 09-05-2004 11:50 PM
Large plasmid cloning b8703126@sinamail.com Protocols and Methods Forum 7 05-10-2004 10:25 PM
Cloning 4.2kb insert in 29.8 fosmid P.C. Protocols and Methods Forum 0 12-01-2003 04:09 AM


All times are GMT. The time now is 01:55 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13843 seconds with 16 queries