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micromolar conversion

micromolar conversion - Molecular Cloning Forum

micromolar conversion - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 04-22-2010, 01:28 PM
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Default micromolar conversion



My primer concentration is 0.1 uM in 10 uL and I need to get 125 ng of this primer.
how many uL should i take from my primer in total reaction of 50 uL.

Is there any online calculator that can help me ???
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Old 04-22-2010, 03:52 PM
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Default Re: micromolar conversion

You need the MW of the primer, should be on tube label or product sheet.

See if you can plug it in to calc'n:

0.1 x10^-6 mol/L x 0.000010L x ___g/mol (MW of primer in g/mol) = ___g in 10ul of primer

Then,

take your X grams primer / 10ul = primer in g/ul
move decimal 9 places to right or multiply times 1x10^-9 to convert to ng/ul
Now take 125ng / conc of primer (ng/ul) and voila get volume in ul that yields 125ng of primer.
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Old 04-23-2010, 11:35 AM
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Default Re: micromolar conversion

Thanx danfive
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Old 04-29-2010, 04:38 PM
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Post Re: micromolar conversion

Quote:
Is the answer to be 14 microlitre if the MW is 8905.
No, can't be MW 8905 is in Da you need g/ml.

If the primer sheet or label doesn't have it, it might have the amount of oligo ex. amount of oligo =12.1OD260 =60.40nMoles =0.38mg

From that you could get grams/mol if you wanted (mg/nMoles convert to g/mol), but you can go straight to 0.38mg divided by volume to make stock solution, say 604ul (100uM stock in example).

You would have 380ug/604ul = 0.63ug/ul ---conversions--->
0.63ug/ul * 1000ul/ml * 1g/1000000ug = 0.000630g/ml
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Old 04-30-2010, 04:23 AM
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Default Re: micromolar conversion

Thanx Danfive
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