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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


ideal temperatures for cloning miRNAs in E coli

ideal temperatures for cloning miRNAs in E coli - Molecular Cloning Forum

ideal temperatures for cloning miRNAs in E coli - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 02-04-2010, 08:26 PM
Pipette Filler
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Default ideal temperatures for cloning miRNAs in E coli



Are there ideal temperatures, i.e., 30 or 32 degrees C, for amplifying E coli that contain plasmids with inserted miRNAs?
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cloning , coli , ideal , mirnas , temperatures


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