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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


FREE DNA Insert Vector Ligation Calculator

FREE DNA Insert Vector Ligation Calculator - Molecular Cloning Forum

FREE DNA Insert Vector Ligation Calculator - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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  #1  
Old 01-20-2010, 03:46 AM
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Lightbulb FREE DNA Insert Vector Ligation Calculator



FREE DNA Insert Vector Ligation Calculator

Very simple and easy to use!!

http://www.molecularstation.com/calc...or-calculator/

Make sure you tell your lab mates! It saves time and creates bacteria clones when incubated
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Old 10-19-2010, 08:58 PM
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Default Re: FREE DNA Insert Vector Ligation Calculator

i need to know simple concpt for gateway clonning with procedure
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Old 11-15-2012, 03:44 AM
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Default Re: FREE DNA Insert Vector Ligation Calculator

Now I have a hard-to-transfection cells.Could anyone knows a kind of transfection reagent wich provides a one-minute transfection experiment operation, in addition, eliminate the need for removal of the complexes or a medium change after the transfection?
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Old 09-16-2013, 11:59 AM
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Default Re: FREE DNA Insert Vector Ligation Calculator

Hi Guys, I would like to know....is that be a problem if your are growing your plasmid in different strain bacteria for cloning?, I mean, if you have bacteria grown in DH5alpha but using it for cloning in XL Blue Competent Cell?

Cheer
San
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