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-   -   FREE DNA Insert Vector Ligation Calculator (http://www.molecularstation.com/forum/molecular-cloning-forum/72273-free-dna-insert-vector-ligation-calculator.html)

admin 01-20-2010 03:46 AM

FREE DNA Insert Vector Ligation Calculator
 
FREE DNA Insert Vector Ligation Calculator

Very simple and easy to use!!

[Only registered and activated users can see links. Click Here To Register...]

Make sure you tell your lab mates! :pumped: It saves time and creates bacteria clones when incubated :microwave:

si73 10-19-2010 08:58 PM

Re: FREE DNA Insert Vector Ligation Calculator
 
i need to know simple concpt for gateway clonning with procedure

Taochi 11-15-2012 03:44 AM

Re: FREE DNA Insert Vector Ligation Calculator
 
Now I have a hard-to-transfection cells.Could anyone knows a kind of transfection reagent wich provides a one-minute transfection experiment operation, in addition, eliminate the need for removal of the complexes or a medium change after the transfection?

San7 09-16-2013 11:59 AM

Re: FREE DNA Insert Vector Ligation Calculator
 
Hi Guys, I would like to know....is that be a problem if your are growing your plasmid in different strain bacteria for cloning?, I mean, if you have bacteria grown in DH5alpha but using it for cloning in XL Blue Competent Cell?

Cheer
San


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