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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


gene-trap retrovirus vector

gene-trap retrovirus vector - Molecular Cloning Forum

gene-trap retrovirus vector - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 01-17-2010, 06:39 PM
Pipette Filler
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Default gene-trap retrovirus vector



Hi, guys! I need to write a report about using of gene-trap retrovirus vectors in haploid human cells to generate null alleles. The insertion of provirus is random in genomic DNA, so how can I make the gene-trap insertion that would be in desire gene, which I want to disrupt? How we understand in which gene is the gene-trap insertion? 10x in advance for help.
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