Problems in screening genomic DNA Library with antibody
I constructed a genomic DNA library of a bacterium using Partial DNA digestion employing Lambda ZAP II Kit (Stratagene). I did everything alright as shown in kit. I used 2-4 kb fragments for ligation with pre-digested vector supplied in kit. I used 1 uL of ligate for packaging and then amplified the library. In Blue-white screening, all the plaques showed transperant or clear with blue rings at the edges. There was no blue colony. I thought all are recombinant clones. Then, I started screening the library using antibodies. But I am not getting any positive clones. Please any one help me and tell the reason? My antibodies are good and I checked them several times.:headbonk:
|All times are GMT. The time now is 08:45 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved