Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Register Search Today's Posts Mark Forums Read

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


confusion about pBBR1mcs expression

confusion about pBBR1mcs expression - Molecular Cloning Forum

confusion about pBBR1mcs expression - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-05-2009, 10:54 AM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Question confusion about pBBR1mcs expression



Hi all, I constructed a gene in pET22b before, later I was told by my advisor to construct the gene in pBBR1mcs,so I digested the pET22b construction with XbaI and XhoI, transferred the rbs site together with my gene into pBBR1mcs plasmid. The gene expressed well without induction, and my advisor told me it is because of the lac promoter. However, today I found the lac promoter is upstream XhoI site in pBBR1mcs! How can it be explained?
Reply With Quote
Reply

Tags
confusion , expression , pbbr1mcs


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
qPCR NEWSLETTER - October 2006 Editor www.Gene-Quantification.info Protocols and Methods Forum 0 10-26-2006 12:25 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 04:34 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16650 seconds with 16 queries