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Cant find the Promoter sequence....

Cant find the Promoter sequence.... - Molecular Cloning Forum

Cant find the Promoter sequence.... - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 08-04-2009, 10:26 PM
Pipette Filler
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Default Cant find the Promoter sequence....



Hi guys im stuck ....i have bee trying to locate a promoter of alpha fetoprotein ....some people have used .3Kb promoter sequence while some have used .2 kb with enhancer.....and in their papers they havent mentioned what sequence they took for .2 kb ..they have mentioned ( 212bp )....3Kb sequence i know..so i am not able to locate the .2 kb sequence.


Also how can i obtain the 5' flanking nucleotide seq of a gene in pubmed??...

help pls
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Old 08-06-2009, 03:36 PM
esl esl is offline
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Default Re: Cant find the Promoter sequence....

If you go do a BLAT search on your sequence (try [Only registered users see links. ]) then you can find the 5' sequence upstream of your submitted region.

Paste your sequence into the query box and submit it (make sure you select the right species).
On the results page, find the sequence which is yours (usually at the top of the list) and click on 'browser'.
On the next display, click on the Gene Name for your sequence.
On the next page, under 'Links to Sequence', select 'Genomic Sequence'
The final page offers retrieval options where you can choose how many bases upstream you would like to see the sequence for.

Good luck!
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